A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.
The neo (neomycin-resistance) gene of transposon Tn5 encodes the enzyme neomycin phosphotransferase II (EC 2.7.1.95), which confers resistance to various aminoglycoside antibiotics, including kanamycin and G418. The gene is widely used as a selectable marker in the transformation of organisms as div...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53915/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2159150/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.9.3435 |
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