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Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens
VirE2 is a ssDNA binding protein essential for virulence in Agrobacterium tumefaciens. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging based on the ReAsH reagent. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretio...
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| 發表在: | PLoS One |
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| Main Authors: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Public Library of Science
2017
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5389803/ https://ncbi.nlm.nih.gov/pubmed/28403156 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0175273 |
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