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Cloning-independent markerless gene editing in Streptococcus sanguinis: novel insights in type IV pilus biology

Streptococcus sanguinis, a naturally competent opportunistic human pathogen, is a Gram-positive workhorse for genomics. It has recently emerged as a model for the study of type IV pili (Tfp)—exceptionally widespread and important prokaryotic filaments. To enhance genetic manipulation of Streptococcu...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Gurung, Ishwori, Berry, Jamie-Lee, Hall, Alexander M. J., Pelicic, Vladimir
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5389465/
https://ncbi.nlm.nih.gov/pubmed/27903891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw1177
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