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Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli.

The SecY protein and other membrane proteins of Escherichia coli were solubilized by mixed micelles of n-octyl beta-D-glucopyranoside, phospholipids, and glycerol. Proteoliposomes formed from this extract by detergent dialysis supported energy-dependent translocation and processing of pro-OmpA. Tran...

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Bibliografische gegevens
Hoofdauteurs: Driessen, A J, Wickner, W
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1990
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC53843/
https://ncbi.nlm.nih.gov/pubmed/2139227
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