Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli.
The SecY protein and other membrane proteins of Escherichia coli were solubilized by mixed micelles of n-octyl beta-D-glucopyranoside, phospholipids, and glycerol. Proteoliposomes formed from this extract by detergent dialysis supported energy-dependent translocation and processing of pro-OmpA. Tran...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53843/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2139227/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.8.3107 |
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