A modified ‘NanoSuit®’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy

Although field-emission scanning electron microscopy (FE-SEM) has proven very useful in biomedical research, the high vacuum required (10(−3) to 10(−7) Pa) precludes direct observations of living cells and tissues at high resolution and often produces unwanted structural changes. We have previously...

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Dettagli Bibliografici
Pubblicato in:R Soc Open Sci
Autori principali: Takaku, Yasuharu, Suzuki, Hiroshi, Kawasaki, Hideya, Ohta, Isao, Ishii, Daisuke, Hirakawa, Satoshi, Tsutsui, Takami, Matsumoto, Haruko, Takehara, Sayuri, Nakane, Chinatsu, Sakaida, Kana, Suzuki, Chiaki, Muranaka, Yoshinori, Kikuchi, Hirotoshi, Konno, Hiroyuki, Shimomura, Masatsugu, Hariyama, Takahiko
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Royal Society Publishing 2017
Soggetti:
Biochemistry and Biophysics
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5383832/
https://ncbi.nlm.nih.gov/pubmed/28405375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsos.160887
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