Seq-Well: A Portable, Low-Cost Platform for High-Throughput Single-Cell RNA-Seq of Low-Input Samples

Single-cell RNA-Seq can precisely resolve cellular states but application to sparse samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively-parallel single-cell RNA-Seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells u...

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Gepubliceerd in:Nat Methods
Hoofdauteurs: Gierahn, Todd M., Wadsworth, Marc H., Hughes, Travis K., Bryson, Bryan D., Butler, Andrew, Satija, Rahul, Fortune, Sarah, Love, J. Christopher, Shalek, Alex K.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2017
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5376227/
https://ncbi.nlm.nih.gov/pubmed/28192419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4179
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