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A method for difference cloning: gene amplification following subtractive hybridization.

We describe a procedure for genomic difference cloning, a method for isolating sequences present in one genomic DNA population ("tester") that is absent in another ("driver"). By subtractive hybridization, a large excess of driver is used to remove sequences common to a biotinyla...

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Bibliografische gegevens
Hoofdauteurs: Wieland, I, Bolger, G, Asouline, G, Wigler, M
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1990
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC53762/
https://ncbi.nlm.nih.gov/pubmed/2138783
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