Determinants of PCR performance (Xpert MTB/RIF), including bacterial load and inhibition, for TB diagnosis using specimens from different body compartments

The determinants of Xpert MTB/RIF sensitivity, a widely used PCR test for the diagnosis of tuberculosis (TB) are poorly understood. We compared culture time-to-positivity (TTP; a surrogate of bacterial load), MTB/RIF TB-specific and internal positive control (IPC)-specific C(T) values, and clinical...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Theron, Grant, Peter, Jonny, Calligaro, Greg, Meldau, Richard, Hanrahan, Colleen, Khalfey, Hoosain, Matinyenya, Brian, Muchinga, Tapuwa, Smith, Liezel, Pandie, Shaheen, Lenders, Laura, Patel, Vinod, Mayosi, Bongani M., Dheda, Keertan
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5375978/
https://ncbi.nlm.nih.gov/pubmed/25014250
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep05658
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