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Probing Hydronium Ion Histidine NH Exchange Rate Constants in the M2 Channel via Indirect Observation of Dipolar-Dephased (15)N Signals in Magic-Angle-Spinning NMR

Water–protein chemical exchange in membrane-bound proteins is an important parameter for understanding how proteins interact with their aqueous environment, but has been difficult to observe in membrane-bound biological systems. Here, we demonstrate the feasibility of probing specific water–protein...

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Detalhes bibliográficos
Publicado no:J Am Chem Soc
Main Authors: Fu, Riqiang, Miao, Yimin, Qin, Huajun, Cross, Timothy A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5368641/
https://ncbi.nlm.nih.gov/pubmed/27960325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b08376
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