Patient-specific three-dimensional explant spheroids derived from human nasal airway epithelium: a simple methodological approach for ex vivo studies of primary ciliary dyskinesia

BACKGROUND: Three-dimensional explant spheroid formation is an ex vivo technique previously used in studies of airway epithelial ion and water transport. Explanted cells and sheets of nasal epithelium form fully differentiated spheroids enclosing a partly fluid-filled lumen with the ciliated apical...

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Detalhes bibliográficos
Publicado no:Cilia
Main Authors: Marthin, June Kehlet, Stevens, Elizabeth Munkebjerg, Larsen, Lars Allan, Christensen, Søren Tvorup, Nielsen, Kim Gjerum
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5364668/
https://ncbi.nlm.nih.gov/pubmed/28344781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13630-017-0049-5
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