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Purification and lipid-layer crystallization of yeast RNA polymerase II.

Yeast RNA polymerase II was purified to homogeneity by a rapid procedure involving immunoaffinity chromatography. The purified enzyme contained 10 subunits, as reported for conventional preparations, but with no detectable proteolysis of the largest subunit. In assays of initiation of transcription...

詳細記述

保存先:
書誌詳細
主要な著者: Edwards, A M, Darst, S A, Feaver, W J, Thompson, N E, Burgess, R R, Kornberg, R D
フォーマット: Artigo
言語:Inglês
出版事項: 1990
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC53638/
https://ncbi.nlm.nih.gov/pubmed/2179949
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