Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

BACKGROUND: The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughp...

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Detalles Bibliográficos
Publicado en:Stand Genomic Sci
Autores principales: Kong, Nguyet, Ng, Whitney, Thao, Kao, Agulto, Regina, Weis, Allison, Kim, Kristi Spittle, Korlach, Jonas, Hickey, Luke, Kelly, Lenore, Lappin, Stephen, Weimer, Bart C.
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2017
Materias:
Standard Operating Procedure
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5363030/
https://ncbi.nlm.nih.gov/pubmed/28344744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40793-017-0239-1
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