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The Cation−π Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification

[Image: see text] The design of fluorogenic probes for a Halo tag is highly desirable but challenging. Previous work achieved this goal by controlling the chemical switch of spirolactones upon the covalent conjugation between the Halo tag and probes or by incorporating a “channel dye” into the subst...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Liu, Yu, Miao, Kun, Dunham, Noah P., Liu, Hongbin, Fares, Matthew, Boal, Amie K., Li, Xiaosong, Zhang, Xin
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2017
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5362743/
https://ncbi.nlm.nih.gov/pubmed/28221782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00056
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