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Construction Strategy for an Internal Amplification Control for Real-Time Diagnostic Assays Using Nucleic Acid Sequence-Based Amplification: Development and Clinical Application

An important analytical control in molecular amplification-based methods is an internal amplification control (IAC), which should be included in each reaction mixture. An IAC is a nontarget nucleic acid sequence which is coamplified simultaneously with the target sequence. With negative results for...

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Main Authors: Rodríguez-Lázaro, David, D'Agostino, Martin, Pla, Maria, Cook, Nigel
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC535304/
https://ncbi.nlm.nih.gov/pubmed/15583319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.12.5832-5836.2004
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