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Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision

We describe here a new approach to marker recycling, a controlled sequence of steps in which a genetic marker is selected and then lost. Marker recycling is important for genetic manipulation, because it allows a single selection marker to be used repeatedly. Our approach relies upon the ability of...

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Detalhes bibliográficos
Publicado no:mSphere
Main Authors: Huang, Manning Y., Mitchell, Aaron P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352831/
https://ncbi.nlm.nih.gov/pubmed/28317025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00050-17
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