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Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in...

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Autori principali: Nielsen, Jeppe L., Schramm, Andreas, Bernhard, Anne E., van den Engh, Gerrit J., Stahl, David A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC535210/
https://ncbi.nlm.nih.gov/pubmed/15574959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7550-7554.2004
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