Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing

Using the proteomic tandem affinity purification (TAP) method, we have purified the Saccharomyces cerevisie U2 snRNP-associated splicing factors SF3a and SF3b. While SF3a purification revealed only the expected subunits Prp9p, Prp11p and Prp21p, yeast SF3b was found to contain only six subunits, inc...

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Main Authors: Dziembowski, Andrzej, Ventura, Ana-Paula, Rutz, Berthold, Caspary, Friederike, Faux, Céline, Halgand, Frédéric, Laprévote, Olivier, Séraphin, Bertrand
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2004
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC535094/
https://ncbi.nlm.nih.gov/pubmed/15565172
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7600482
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