Insights into ligand binding to a Glutathione S-transferase from mango: structure, thermodynamics and kinetics

We studied a mango glutathione S-transferase (GST) (Mangifera indica) bound to glutathione (GSH) and S-hexyl glutathione (GSX). This GST Tau class (MiGSTU) had a molecular mass of 25.5 kDa. MiGSTU Michaelis-Menten kinetic constants were determined for their substrates obtaining a K(m), V(max) and k(...

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Publicado en:Biochimie
Main Authors: Valenzuela-Chavira, Ignacio, Contreras-Vergara, Carmen A., Arvizu-Flores, Aldo A., Serrano-Posada, Hugo, Lopez-Zavala, Alonso A., García-Orozco, Karina D., Hernandez-Paredes, Javier, Rudiño-Piñera, Enrique, Stojanoff, Vivian, Sotelo-Mundo, Rogerio R., Islas-Osuna, Maria A.
Formato: Artigo
Idioma:Inglês
Publicado: 2017
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5346462/
https://ncbi.nlm.nih.gov/pubmed/28104507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.biochi.2017.01.005
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