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Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

Mammalian DNA segments cloned as yeast artificial chromosomes (YACs) can be manipulated by DNA-mediated transformation when placed in an appropriate yeast genetic background. A "fragmenting vector" has been developed that can introduce a yeast telomere and selectable marker into human-deri...

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Autori principali: Pavan, W J, Hieter, P, Reeves, R H
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1990
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC53462/
https://ncbi.nlm.nih.gov/pubmed/2406718
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