RNA processing and ribonucleoprotein assembly studied in vivo by RNA transfection.
We present a method for studying RNA processing and ribonucleoprotein assembly in vivo, by using RNA synthesized in vitro. SP6-transcribed 32P-labeled U2 small nuclear RNA precursor molecules were introduced into cultured human 293 cells by calcium phosphate-mediated uptake, as in standard DNA trans...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1990
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53458/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2137610/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.4.1283 |
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