High-frequency nuclear transformation of Chlamydomonas reinhardtii.
By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. The nitrate reductase gene from wi...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53444/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2105499/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.3.1228 |
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