Efficient and reliable establishment of lymphoblastoid cell lines by Epstein-Barr virus transformation from a limited amount of peripheral blood

Lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus (EBV) serve as an unlimited resource of human genomic DNA. The protocol that is widely used to establish LCLs involves peripheral blood mononuclear cell isolation by density gradient centrifugation, however, that method requires as m...

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Vydáno v:Sci Rep
Hlavní autoři: Omi, Natsue, Tokuda, Yuichi, Ikeda, Yoko, Ueno, Morio, Mori, Kazuhiko, Sotozono, Chie, Kinoshita, Shigeru, Nakano, Masakazu, Tashiro, Kei
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2017
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5341036/
https://ncbi.nlm.nih.gov/pubmed/28272413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep43833
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