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ISOLATION OF ADENYL CYCLASE FROM Escherichia coli

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Tao, Mariano, Lipmann, Fritz
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1969
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC534038/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4897910/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.63.1.86
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