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An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs

Cryogenic storage of sperm from genetically altered Xenopus improves cost effectiveness and animal welfare associated with their use in research; currently it is routine for X. tropicalis but not reliable for X. laevis. Here we compare directly the three published protocols for Xenopus sperm freeze-...

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Detalhes bibliográficos
Publicado no:Theriogenology
Main Authors: Pearl, Esther, Morrow, Sean, Noble, Anna, Lerebours, Adelaide, Horb, Marko, Guille, Matthew
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5340284/
https://ncbi.nlm.nih.gov/pubmed/28237331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.theriogenology.2017.01.007
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