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An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs

Cryogenic storage of sperm from genetically altered Xenopus improves cost effectiveness and animal welfare associated with their use in research; currently it is routine for X. tropicalis but not reliable for X. laevis. Here we compare directly the three published protocols for Xenopus sperm freeze-...

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Vydáno v:Theriogenology
Hlavní autoři: Pearl, Esther, Morrow, Sean, Noble, Anna, Lerebours, Adelaide, Horb, Marko, Guille, Matthew
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5340284/
https://ncbi.nlm.nih.gov/pubmed/28237331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.theriogenology.2017.01.007
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