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Identifying ionic interactions within a membrane using BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions

The assembly of integral membrane protein complexes is frequently supported by transmembrane domain (TMD) interactions. Here, we present the BLaTM assay that measures homotypic as well as heterotypic TMD-TMD interactions in a bacterial membrane. The system is based on complementation of β-lactamase...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Schanzenbach, Christoph, Schmidt, Fabian C., Breckner, Patrick, Teese, Mark G., Langosch, Dieter
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5339904/
https://ncbi.nlm.nih.gov/pubmed/28266525
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep43476
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