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Total chemical synthesis of a thermostable enzyme capable of polymerase chain reaction
Polymerase chain reaction (PCR) has been a defining tool in modern biology. Towards realizing mirror-image PCR, we have designed and chemically synthesized a mutant version of the 352-residue thermostable Sulfolobus solfataricus P2 DNA polymerase IV with l-amino acids and tested its PCR activity bio...
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| Publicado no: | Cell Discov |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5335361/ https://ncbi.nlm.nih.gov/pubmed/28265464 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/celldisc.2017.8 |
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