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Converting Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) to a regioselective α2–6-sialyltransferase by saturation mutagenesis and regioselective screening()
A microtiter plate-based screening assay capable of determining the activity and regioselectivity of sialyltransferases was developed. This assay was used to screen two single-site saturation libraries of Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) for α2–6-sialyltransferase activity and...
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| Udgivet i: | Org Biomol Chem |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5333693/ https://ncbi.nlm.nih.gov/pubmed/28134951 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6ob02702d |
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