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Converting Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) to a regioselective α2–6-sialyltransferase by saturation mutagenesis and regioselective screening()

A microtiter plate-based screening assay capable of determining the activity and regioselectivity of sialyltransferases was developed. This assay was used to screen two single-site saturation libraries of Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) for α2–6-sialyltransferase activity and...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Org Biomol Chem
Prif Awduron: McArthur, John B., Yu, Hai, Zeng, Jie, Chen, Xi
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5333693/
https://ncbi.nlm.nih.gov/pubmed/28134951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6ob02702d
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