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Gut Microbiota Analysis Results Are Highly Dependent on the 16S rRNA Gene Target Region, Whereas the Impact of DNA Extraction Is Minor

Next-generation sequencing (NGS) is currently the method of choice for analyzing gut microbiota composition. As gut microbiota composition is a potential future target for clinical diagnostics, it is of utmost importance to enhance and optimize the NGS analysis procedures. Here, we have analyzed the...

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Dettagli Bibliografici
Pubblicato in:J Biomol Tech
Autori principali: Rintala, Anniina, Pietilä, Sami, Munukka, Eveliina, Eerola, Erkki, Pursiheimo, Juha-Pekka, Laiho, Asta, Pekkala, Satu, Huovinen, Pentti
Natura: Artigo
Lingua:Inglês
Pubblicazione: Association of Biomolecular Resource Facilities 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5330390/
https://ncbi.nlm.nih.gov/pubmed/28260999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.17-2801-003
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