PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli

We recently demonstrated that the Escherichia coli ribosome is robust enough to accommodate foreign 16S rRNAs from diverse gamma- and betaproteobacteria bacteria (Kitahara et al., 2012). Therein, we used the common universal primers Bac8f and UN1541r to obtain a nearly full-length gene. However, we...

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Bibliografiske detaljer
Udgivet i:Front Bioeng Biotechnol
Main Authors: Miyazaki, Kentaro, Sato, Mitsuharu, Tsukuda, Miyuki
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2017
Fag:
Bioengineering and Biotechnology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5329695/
https://ncbi.nlm.nih.gov/pubmed/28293553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2017.00014
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