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Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity
MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging. We have used a combination of protein design and directed evolution to develop mCherry variants with low cytotoxicity to E...
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Publicado no: | PLoS One |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Public Library of Science
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5328254/ https://ncbi.nlm.nih.gov/pubmed/28241009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0171257 |
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