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Impact of heat shock step on bacterial transformation efficiency

CaCl(2) treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl(2 )treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL...

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Detalhes bibliográficos
Publicado no:Mol Biol Res Commun
Main Authors: Rahimzadeh, Maral, Sadeghizadeh, Majid, Najafi, Farhood, Arab, Seyed, Mobasheri, Hamid
Formato: Artigo
Idioma:Inglês
Publicado em: Shiraz University 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5326489/
https://ncbi.nlm.nih.gov/pubmed/28261629
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