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Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis

We report optimized methods for preparing actin-intact Xenopus egg extract. This extract is minimally perturbed, undiluted egg cytoplasm where the cell cycle can be experimentally controlled. It contains abundant organelles and glycogen, and supports active metabolism and cytoskeletal dynamics that...

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Bibliografske podrobnosti
izdano v:Methods Cell Biol
Main Authors: Field, Christine M., Pelletier, James F., Mitchison, Timothy J.
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5322469/
https://ncbi.nlm.nih.gov/pubmed/28065319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2016.04.014
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