Expression of a cloned streptavidin gene in Escherichia coli.
We describe the construction of systems for expressing the cloned streptavidin gene in Escherichia coli. Although the streptavidin gene is extremely lethal to the host cells, because of the strong biotin binding of the gene product, the gene was expressed efficiently in E. coli by using T7 RNA polym...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53216/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2404273/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.1.142 |
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