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Isolation of Latex Bead Phagosomes from Dictyostelium for in vitro Functional Assays
We describe a protocol to purify latex bead phagosomes (LBPs) from Dictyostelium cells. These can be later used for various in vitro functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on in vitro polymerized microtubules. Phagosomes are allowed to...
שמור ב:
| הוצא לאור ב: | Bio Protoc |
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| Main Authors: | , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
2016
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5321523/ https://ncbi.nlm.nih.gov/pubmed/28239623 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2056 |
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