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Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells
Single-cell microscopy provides a powerful tool to visualize cellular and subcellular processes in wild-type and mutant cells by observing fluorescently tagged proteins. Here, we describe three simple methods to visualize fission yeast cells: gelatin slides, coverslip-bottom dishes, and tetrad fluor...
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Publicado no: | Methods Mol Biol |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5312653/ https://ncbi.nlm.nih.gov/pubmed/26519302 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3145-3_2 |
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