Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor

AIM: To investigate a suitable long-term culture system and optimal cryopreservation of intestinal organoid to improve organoid-based therapy by acquiring large numbers of cells. METHODS: Crypts were isolated from jejunum of C57BL/6 mouse. Two hundred crypts were cultured in organoid medium with eit...

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Vydáno v:World J Gastroenterol
Hlavní autoři: Han, Sung-Hoon, Shim, Sehwan, Kim, Min-Jung, Shin, Hye-Yun, Jang, Won-Suk, Lee, Sun-Joo, Jin, Young-Woo, Lee, Seung-Sook, Lee, Seung Bum, Park, Sunhoo
Médium: Artigo
Jazyk:Inglês
Vydáno: Baishideng Publishing Group Inc 2017
Témata:
Basic Study
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5311106/
https://ncbi.nlm.nih.gov/pubmed/28246470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v23.i6.964
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