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Development of a high-throughput method to evaluate serum bactericidal activity using bacterial ATP measurement as survival readout

Serum Bactericidal Activity (SBA) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies. To perform a typical SBA assay, serial dilutions of sera are incubated with target bacterial strains and complement. The conventi...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Necchi, Francesca, Saul, Allan, Rondini, Simona
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5305226/
https://ncbi.nlm.nih.gov/pubmed/28192483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0172163
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