Approach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments

In this study, we describe a nested PCR-DGGE strategy to detect Legionella communities from river water samples. The nearly full-length 16S rRNA gene was amplified using bacterial primer in the first step. After, the amplicons were employed as DNA templates in the second PCR using Legionella specifi...

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Dettagli Bibliografici
Pubblicato in:PLoS One
Autori principali: Huang, Wen-Chien, Tsai, Hsin-Chi, Tao, Chi-Wei, Chen, Jung-Sheng, Shih, Yi-Jia, Kao, Po-Min, Huang, Tung-Yi, Hsu, Bing-Mu
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2017
Soggetti:
Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5293244/
https://ncbi.nlm.nih.gov/pubmed/28166249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0170992
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