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Determination of NTPase activities from measurement of true inorganic phosphate in the presence of labile phosphate compounds

One of the most common assays for NTPase activity entails the quantification of inorganic phosphate (P(i)) as a colored phosphomolybdate complex at low pH. While this assay is very sensitive, it is not selective for P(i) in the presence of labile organic phosphate compounds (OPCs). Since NTPase acti...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Katz, Faith E. H., Shi, Xinying, Owens, Cedric P., Joseph, Simpson, Tezcan, F. Akif
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5291744/
https://ncbi.nlm.nih.gov/pubmed/28017740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.12.012
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