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A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system

Dictyostelium discoideum has proven an exceptionally powerful system for studying numerous aspects of cellular and developmental functions. The relatively small (∼34 Mb) chromosomal genome of Dictyostelium and high efficiency of targeted gene disruption have enabled researchers to characterize many...

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Main Authors: Faix, Jan, Kreppel, Lisa, Shaulsky, Gad, Schleicher, Michael, Kimmel, Alan R.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC528815/
https://ncbi.nlm.nih.gov/pubmed/15507682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh136
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