piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice

CRISPR/Cas9 is becoming an increasingly important tool to functionally annotate genomes. However, because genome-wide CRISPR libraries are mostly constructed in lentiviral vectors, in vivo applications are severely limited as a result of difficulties in delivery. Here, we examined the piggyBac (PB)...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Xu, Chunlong, Qi, Xiaolan, Du, Xuguang, Zou, Huiying, Gao, Fei, Feng, Tao, Lu, Hengxing, Li, Shenglan, An, Xiaomeng, Zhang, Lijun, Wu, Yuanyuan, Liu, Ying, Li, Ning, Capecchi, Mario R., Wu, Sen
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 2017
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5278437/
https://ncbi.nlm.nih.gov/pubmed/28062688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1615735114
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