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CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast

Over-expression of a gene by increasing its copy number is often desirable in the model yeast Saccharomyces cerevisiae. It may facilitate elucidation of enzyme functions, and in cell factory design it is used to increase production of proteins and metabolites. Current methods are typically exploitin...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Strucko, Tomas, Buron, Line Due, Jarczynska, Zofia Dorota, Nødvig, Christina Spuur, Mølgaard, Louise, Halkier, Barbara Ann, Mortensen, Uffe Hasbro
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5278378/
https://ncbi.nlm.nih.gov/pubmed/28134264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep41431
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