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Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner

We describe highly effective adhesion culture of human pluripotent stem cells (hPSCs) using laminin fragments without precoating. Culture substrates have been generally thought to exert a cell adhesion effect when they are precoated onto culture vessels. However, simple addition of laminin fragments...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Miyazaki, Takamichi, Isobe, Takehisa, Nakatsuji, Norio, Suemori, Hirofumi
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5278368/
https://ncbi.nlm.nih.gov/pubmed/28134277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep41165
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