Transformation of Tetrahymena to cycloheximide resistance with a ribosomal protein gene through sequence replacement.
A method for transforming Tetrahymena has been established earlier, but its application has been limited because of the lack of selectable markers other than the rRNA-encoding DNA (rDNA). Mutations in the yeast ribosomal protein L29 gene (CYH2) are known that confer cycloheximide resistance. We have...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52744/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1946363/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.21.9493 |
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