Mutational analysis of the Escherichia coli glpFK region with Tn5 mutagenesis and the polymerase chain reaction.

Transposon Tn5 mutagenesis of the Escherichia coli chromosome was used to isolate 21 independent insertion mutations conferring an altered colony color phenotype on MacConkey-glycerol plates. The polymerase chain reaction was used to map 16 of these Tn5 insertions within the glpFK region at 88 min....

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Detalhes bibliográficos
Main Authors: Lupski, J R, Zhang, Y H, Rieger, M, Minter, M, Hsu, B, Ooi, B G, Koeuth, T, McCabe, E R
Formato: Artigo
Idioma:Inglês
Publicado em: 1990
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC526940/
https://ncbi.nlm.nih.gov/pubmed/2170343
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