Mutations that affect Tn5 insertion into pBR322: importance of local DNA supercoiling.
The major hot spot of transposon Tn5 insertion in plasmid pBR322 (hot spot I) is in the promoter for the tetracycline resistance gene (tet). We made a series of pBR322 derivatives with mutations in and around this promoter and assayed their effects on insertion of Tn5 into hot spot I. Those mutation...
Shranjeno v:
| izdano v: | J Bacteriol |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1990
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC526917/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2170333/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.10.5956-5960.1990 |
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