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Cloning and characterization of RNA polymerase core subunits of Chlamydia trachomatis by using the polymerase chain reaction.

Taking advantage of sequence conservation of portions of the alpha, beta, and beta' subunits of RNA polymerase of bacteria and plant chloroplasts, we have designed degenerate oligonucleotides corresponding to these domains and used these synthetic DNA sequences as primers in a polymerase chain react...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Engel, J N, Pollack, J, Malik, F, Ganem, D
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1990
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC526889/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2211507/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.10.5732-5741.1990
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