Purification of the Escherichia coli purine regulon repressor and identification of corepressors.
The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was req...
में बचाया:
| में प्रकाशित: | J Bacteriol |
|---|---|
| मुख्य लेखकों: | , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
American Society for Microbiology (ASM)
1990
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC526876/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2211500/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.10.5637-5642.1990 |
| टैग: |
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