Assay and purification of S-adenosyl-L-methionine:precorrin-2 methyltransferase from Pseudomonas denitrificans.

S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionat...

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Bibliographic Details
Main Authors: Thibaut, D, Couder, M, Crouzet, J, Debussche, L, Cameron, B, Blanche, F
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC526806/
https://ncbi.nlm.nih.gov/pubmed/2172210
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