Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

Vaccinia virus has been used as a vector to express foreign genes for the production of functional and posttranslationally modified proteins. A procedure is described here that allows the rapid native purification of vaccinia-expressed proteins fused to an amino-terminal tag of six histidines. Extra...

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Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Janknecht, R, de Martynoff, G, Lou, J, Hipskind, R A, Nordheim, A, Stunnenberg, H G
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 1991
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52633/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1924358/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.20.8972
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